Evaluation of programmed cell death processes on the lens epithelium of older dogs with diabetic and hypermature cataracts

  • Ana Paula Franco do Amaral Hvenegaard Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Serviço de Oftalmologia
  • Paulo Sergio de Moraes Barros Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Serviço de Oftalmologia
  • Angélica Mendonça Vaz Safatle Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Serviço de Oftalmologia
  • Michelle Barbosa Pereira Braga-Sá Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Serviço de Oftalmologia
  • Luana Vicente Melo Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédica, Programa de Desenvolvimento Celular
  • Ana Carolina Santana Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédica, Programa de Desenvolvimento Celular
  • Bryan Hudson Hossy Universidade Federal do Rio de Janeiro, Clínica Médica, Serviço de Dermatologia
  • Nadia Campos de Oliveira Miguel Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédica, Programa de Desenvolvimento Celular
Keywords: Cataracts, Diabetes, Apoptosis, Autophagy, Lens epithelium cells

Abstract

It is well known that posterior capsule opacification (PCO), one of the most common late postoperative complications of cataract surgery, is mainly caused by proliferation and differentiation of remaining lens epithelial cells (LECs) on the posterior lens capsule. Many authors suggest that alterations induced by the pathophysiology of cataracts, its metabolism and the use of 0.1% trypan blue (TB) must cause some degree of cellular damage on these cells, wicht would help to prevent and/or reduce the incidence of PCO after cataract surgery in humans. Therefore, the aim of this study was to evaluate the expression of cell death markers on LECs of older dogs with diabetic and hypermature cataracts, after capsulorhexis, both using 0.1% TB. Twenty samples collected from 13 dogs of different breeds, with ages varying from 8 to 12 years-old, with diabetic and hypermature cataracts, which had been subjected to phacoemulsification surgery (Phaco) using 0.1% TB for staining were studied. Animals were classified as dogs with diabetic (DC) and hypermature cataracts (HC), and expression of molecular markers for apoptosis and autophagy (caspase-3 and beclin-1) on LECs were obtained by immunofluorescence technique. The expression of caspase-3 and beclin-1 was observed in every studied sample and did not differ between groups. In conclusion, our findings suggest that apoptosis and autophagy processes occur to LECs in older dogs presenting diabetic and hypermature cataracts after Phaco utilizing 0.1% TB. Our results may be helpful to future studies of PCO in post-phacoemulsification surgery patients.

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Published
2018-07-26
How to Cite
Hvenegaard, A. P., Barros, P. S., Safatle, A., Braga-Sá, M., Melo, L., Santana, A. C., Hossy, B. H., & Miguel, N. (2018). Evaluation of programmed cell death processes on the lens epithelium of older dogs with diabetic and hypermature cataracts. Brazilian Journal of Veterinary Research and Animal Science, 55(2), e133668. https://doi.org/10.11606/issn.1678-4456.bjvras.2018.133668
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