Effect of injectable progesterone on follicular development in lactating beef cows treated with estradiol plus a low-concentration progesterone device

  • Luiz Francisco Machado Pfeifer Empresa Brasileira de Pesquisa Agropecuária
  • Reuben John Mapletoft University of Saskatchewan
  • Dinesh Dardawal University of Saskatchewan
  • Jaswant Singh University of Saskatchewan
Keywords: Dominant follicle, Beef cattle, Ovulation, Livestock

Abstract

The effect of injectable progesterone was evaluated along with estradiol benzoate (EB) on the fate of the dominant follicle (DF) present in the ovary at the beginning of low progesterone-based TAI protocol. All cattle were given 500 µg cloprostenol im (PGF; Schering-Plough Animal Health for Estrumate, Pointe-Claire, QC, Canada) twice, 11 d apart, and allocated into two groups: Estradiol group (E group, n = 11) and Estradiol-Progesterone group (EP group, n = 11). Ten days after the second PGF (Day 0), all cattle were given an intravaginal progesterone device with half progesterone concentration (Cue-Mate with a single pod containing 0.78 g progesterone). Concurrently, all cattle were given 1.5 mg im of estradiol benzoate in 3 mL of canola oil and PGF im on Day 0 of the protocol in a crossover design, in which each cow received both treatments. Cows in the EP group also received 100 mg im progesterone (Sigma) in 2 mL of canola oil. On Day 8, progesterone devices were removed and all cattle were given PGF im. All statistical analyses were performed with SAS 9.0. The DF present on Day 0 ovulated in 76% (16/21) of cows from E group and 28.6% (6/21) of cows from EP group (P = 0.002). After progesterone device removal, the size of ovulatory follicle did not differ between groups (E group, 15.5 ± 0.43 mm vs EP group, 15.8 ± 0.98 mm; P = 0.82). These follicles ovulated in 81.3 ± 3.1 h in E group and 71.0 ± 6.1 h in EP group (P = 0.13). In conclusion, injectable progesterone reduced the proportion of cows that ovulate the dominant follicle present in the ovary at the beginning of estradiol-progesterone-based protocols. However, no difference was detected on time of ovulation after progesterone device removal between groups.

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Published
2018-07-26
How to Cite
Pfeifer, L. F., Mapletoft, R. J., Dardawal, D., & Singh, J. (2018). Effect of injectable progesterone on follicular development in lactating beef cows treated with estradiol plus a low-concentration progesterone device. Brazilian Journal of Veterinary Research and Animal Science, 55(2), e136924. https://doi.org/10.11606/issn.1678-4456.bjvras.2018.136924
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