Influence of anestrus and diestrus stages of oocyte on meiotic competence in bitches

  • Leda Maria Costa Pereira Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal e Radiologia Veterinária
  • Paulo Ricardo de Oliveira Bersano Universidade Estadual do Ceará, Faculdade de Veterinária Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina, Centro de Estudos em Venenos de Animais Peçonhentos
  • Alfredo Feio da Maia Lima Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Cirurgia e Anestesiologia Veterinária
  • José Carlos de Figueiredo Pantoja Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Higiene Veterinária e Saúde Pública
  • Maria Denise Lopes Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal e Radiologia Veterinária
Keywords: Estrous cycle, Maturation, Reproductive stage, Bitch

Abstract

The bitch has reproductive peculiarities that differentiate it from other species. Several experiments have been conducted to establish efficient protocols for maturation; however, the results appear to be unsatisfactory. In this respect, the reproductive female donor should be considered, since it can be a factor of variability of findings in this species. The objective of this study was to evaluate the relationship between the estrous cycle phases – phase diestrus and anestrus on canine oocyte in vitro maturation (IVM). The ovaries were transported in sodium chloride 0.9% solution, and were cut into phosphate-buffered saline (PBS) and the cumulus-oocyte complexes (COCs) selected in tissue culture medium (TCM), supplemented with 199 HEPES. A total of 469 grade 1 oocytes were collected from bitches in anestrus and diestrus. These selected oocytes were transferred to the maturation medium for a period of 72 hours, then subjected to hyaluronidase solution and stained with Hoechst 33342 to assess nuclear configuration. The comparison of anestrus and diestrus phase showed no differences (p > 0.05) between the nuclear maturation stages. Thus, the phase of the estrous cycle did not influence the in vitro maturation of canine oocytes, increasing the rates of M-II in this species.

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Published
2015-09-30
How to Cite
Pereira, L., Bersano, P. R., Lima, A., Pantoja, J. C., & Lopes, M. D. (2015). Influence of anestrus and diestrus stages of oocyte on meiotic competence in bitches. Brazilian Journal of Veterinary Research and Animal Science, 52(3), 266-272. https://doi.org/10.11606/issn.1678-4456.v52i3p266-272
Section
ARTICLES