Cytotoxicity of substances leached from a root canal sealer based on mineral trioxide aggregate

Authors

  • Roberta Souza D`Almeida Couto Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP
  • Sueli Patricia Harumi Miyagi Departament of Endodontics, School of Dentistry, Braz Cubas University, Mogi das Cruzes, SP
  • Maria Stella Moreira Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP
  • José Ricardo Archilla Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP
  • Márcia Martins Marques Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.2357-8041.clrd.2014.63160

Keywords:

Endodontics, Cell Culture Techniques, Biocompatible Materials.

Abstract

Aim: Based on its biological and physicochemical characteristics, mineral trioxide aggregate (MTA) could be considered the most appropriate material for root canal obturation; nevertheless, handling of MTA is not easy. The MTA Fillapex (MTA-F) was created in an attempt to combine the physicochemical properties of a root canal sealer with the biological properties of MTA. However, the studies on the biological characteristics of MTA-F are still controversial. Thus, this study aimed to analyze the cytotoxicity of MTA-F. Materials and Methods: Cultured human gingival fi broblasts were grown in Dulbecco’s modifi ed Eagle Medium (DMEM) and submitted to a cell culture medium conditioned by MTA or MTA-F. The conditioned medium contained substances leached from the root canal sealers. Cells grown on a fresh medium served as a positive control. Cell viability was assessed by MTT assay at 1, 3, 5 and 7 days. Data was compared by ANOVA followed by Tukey’s test (p < 0.05). Results: Cells submitted to media conditioned by MTA presented a cell growth curve similar to that of the control cells. For the MTA-F group, cell growth was not observed and cell viability was signifi cantly lower than for the other groups during the entire experiment. Conclusion: Substances leached from MTA-F did not allow cell growth, indicating that this MTA-based root sealer is highly cytotoxic. The biocompatibility characteristic of MTA can be lost with MTA-F, and may compromise the endodontic treatment outcome.

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References

Cavalcanti BN, Rode S de M, França CM, Marques MM. Pulp capping materials exert an effect on the secretion of IL-1β and IL-8 by migrating human neutrophils. Braz Oral Res. 2011Jan-Feb;25(1):13-8.

Barkhordar RA, Ghani QP, Russell TR, Hussain MZ. Interleukin-1beta activity and collagen synthesis in human dental pulp fibroblasts. J Endod. 2002 Mar;28(3):157-9.

Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review-part III: Clinical applications, drawbacks, and mechanism of action. J Endod. 2010 Mar;36(3):400-13. doi: 10.1016/j.joen.2009.09.009.

Mente J, Hage N, Pfefferle T, Koch MJ, Geletneky B, Dreyhaupt J, et al. Treatment outcome of mineral trioxide aggregate: repair of root perforations. J Endod. 2010 Feb;36(2):208-13.

doi: 10.1016/j.joen.2009.10.012. Epub 2009 Dec 16.

Damle SG, Bhattal H, Loomba A. Apexification of anterior teeth: a comparative evaluation of mineral trioxide aggregate and calcium hydroxide paste. J Clin Pediatr Dent. 2012 Spring;36(3):263-8.

Torabinejad M, Hong CU, McDonald F, Pitt Ford TR. Physical and chemical properties of a new root-end filling material. J Endod. 1995 Jul;21(7):349-53.

Fridland M, Rosado R. Mineral trioxide aggregate (MTA) solubility and porosity with different water-to-powder ratios. J Endod. 2003 Dec;29(12):814-7.

Camilleri J, Montesin FE, Brady K, Sweeney R, Curtis RV, Ford TR. The constitution of mineral trioxide aggregate. Dent Mater. 2005 Apr;21(4):297-303. Epub 2007 Jul 19.

Gomes-Filho JE, Moreira JV, Watanabe S, Lodi CS, Cintra LT, Dezan Junior E, et al. Sealability of MTA and calcium hydroxide containing sealers. J Appl Oral Sci. 2012 May-Jun;20(3):347-51.

Mozayeni MA, Milani AS, Marvasti LA, Asgary S. Cytotoxicity of calcium enriched mixture cement compared with mineral trioxide aggregate and intermediate restorative material. Aust Endod J. 2012;38:70-75. doi: 10.1111/j.1747-4477.2010.00269.x. Epub 2010 Oct 24.

Parirokh M, Torabinejad M. Mineral Trioxide Aggregate: a comprehensive literature review-part I: chemical, physical, and antibacterial properties. J Endod. 2010 Jan;36(1):16-27. doi: 10.1016/j.joen.2009.09.006.

Al-Hiyasat AS, Tayyar M, Darmani H. Cytotoxicity evaluation of various resin based root canal sealers. Int Endod J. 2010 Feb;43(2):148-53. doi: 10.1111/j.1365-2591.2009.01669.x.

Gomes-Filho JE, Watanabe S, Lodi CS, Cintra LT, Nery MJ, Filho JA, et al. Rat tissue reaction to MTA FILLAPEX(). Dent Traumatol. 2012 Dec;28(6):452-6. doi: 10.1111/j.1600-9657.2011.01096.x. Epub 2011 Dec 8.

Zmener O, Martinez Lalis R, Pameijer CH, Chaves C, Kokubu G, Grana D. Reaction of rat subcutaneous connective tissue to a mineral trioxide aggregate–based and a zinc oxide and eugenol sealer. J Endod. 2012 Sep;38(9):1233-8. doi: 10.1016/j.joen.2012.05.010. Epub 2012 Jul 6.

Scelza MZ, Linhares AB, da Silva LE, Granjeiro JM, Alves GG. A multiparametric assay to compare the cytotoxicity of endodontic sealers with primary human osteoblasts. Int Endod J. 2011 Jan;45(1):12-8. doi: 10.1111/j.1365-2591.2011.01941.x. Epub 2011 Sep 8.

Bin CV, Valera MC, Camargo SE, Rabelo SB, Silva GO, Balducci I, et al. Cytotoxicity and genotoxicity of root canal sealers based on mineral trioxide aggregate. J Endod. 2012 Apr;38(4):495-500. doi: 10.1016/j.joen.2011.11.003. Epub 2011 Dec 23.

Salles LP, Gomes-Cornélio AL, Guimarães FC, Herrera BS, Bao SN, Rossa-Junior C, et al. Mineral trioxide aggregate-based endodontic sealer stimulates hydroxyapatite nucleation in human osteoblast-like cell culture. J Endod. 2012 Jul;38(7):971-6. doi: 10.1016/j.joen.2012.02.018. Epub 2012 Apr 6.

Silva EJNL, Rosa TP, Herrera DR, Jacinto RC, Gomes BPFA, Zaia AA. Evaluation of Cytotoxicity and physicochemical properties of calcium silicate-based endodontic sealer MTA Fillapex. J Endod. 2013 Feb;39(2):274-7. doi: 10.1016/j.joen.2012.06.030. Epub 2012 Aug 9.

Yoshino P, Nishiyama CK, Modena KC, Santos CF, Sipert CR. In vitro cytotoxicity of white MTA, MTA Fillapex and Portland cement on human periodontal ligament fibroblasts. Braz Dent J. 2013;24(2):111-6. doi: 10.1590/0103-6440201302115.

Silva EJ, Santos CC, Zaia AA. Long-term cytotoxic effects of contemporary root canal sealers. J Appl Oral Sci. 2013 Jan-Feb;21(1):43-7.

Güven EP, Yalvaç ME, Kayahan MB, Sunay H, Şahın F, Bayirli G. Human tooth germ stem cell response to calciumsilicate based endodontic cements. J Appl Oral Sci. 2013 Jul-Aug;21(4):351-7. doi: 10.1590/1678-775720130047.

International Organization for Standardization ISO10993-5: biological evaluation of medical devices - Part 5: tests for in vitro cytotoxicity. [place unknown]: ISO; 2009.

Torabinejad M, Parirokh M. Mineral trioxide aggregate: a comprehensive literature review-part II: leakage and biocompatibility investigations. J Endod. 2010 Feb;36(2):190-202. doi: 10.1016/j.joen.2009.09.010.

Viola NV, Guerreiro-Tanomaru JM, da Silva GF, Sasso-Cerri E, Tanomaru-Filho M, Cerri PS. Biocompatibility of an experimental MTA sealer implanted in the rat subcutaneous: quantitative and immunohistochemical evaluation. J Biomed Mater Res B Appl Biomater. 2012 Oct;100B(7):1773-81. doi:

1002/jbm.b.32744. Epub 2012 Jul 23.

Cintra LT, Ribeiro TA, Gomes-Filho JE, Bernabé PF, Watanabe S, Facundo AC, et al. Biocompatibility and biomineralization assessment of a new root canal sealer and root-end filling material. Dent Traumatol. 2013 Apr;29(2):145-50. doi: 10.1111/j.1600-9657.2012.01142.x. Epub 2012 Apr 18.

Annamalai S, Mungara J. Efficacy of mineral trioxide aggregate as an apical plug in non-vital young permanent teeth: preliminary results. J Clin Pediatr Dent. 2010 Winter; 35(2):149-55.

Kqiku L, Ebeleseder KA, Glockner K. Treatment of invasive cervical resorption with sandwich technique using mineral trioxide aggregate: a case report. Oper Dent. 2012 Jan-Feb;37(1):98-106. doi: 10.2341/11-143-S. Epub 2011 Sep 27.

Torabinejad M, Pitt Ford TR. Root end filling materials: a review. Endod Dent Traumatol. 1996 Aug;12(4):161-78.

Sritharan A. Discuss that the coronal seal is more important than the apical seal for endodontic success. Aust Endod J. 2002 Dec;28(3):112-5.

Song M, Kim E. A prospective randomized controlled study of mineral trioxide aggregate and super ethoxy-benzoic acid as root-end filling materials in endodontic microsurgery. J Endod. 2012 Jul;38(7):875-9. doi: 10.1016/j.joen.2012.04.008. Epub 2012 May 16.

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Published

2014-09-08

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Original Research