Exopolysaccharides from Klebsiella oxytoca: anti-inflammatory activity
DOI:
https://doi.org/10.1590/s2175-97902022e190511%20Keywords:
Polysaccharides, Inflammation, Pleurisy, Edema, Leukocyte migration, Klebsiella oxytocaAbstract
Exopolysaccharides (EPS) produced by Klebsiella oxytoca are of environmental, pharmaceutical,
and medicinal interest. However, studies about the anti-inflammatory activity of EPS produced by
this microorganism still remain limited. The aim of this study was to produce, characterize, and
evaluate the anti-inflammatory activity of EPS from K. oxytoca in a pleurisy model. Colorimetric
analysis revealed that precipitated crude exopolysaccharides (KEPSC) and deproteinated
exopolysaccharides (KEPS) present high levels of total carbohydrates (65.57% and 62.82%,
respectively). Analyses of uronic acid (7.90% in KEPSC and 6.21% in KEPS) and pyruvic acid
(3.01% in KEPSC and 1.68% in KEPS) confirm that the EPS are acidic. Gas chromatographymass spectrometry analyses demonstrated that the EPS consisted of rhamnose (29.83%), glucose
(11.21%), galactose (52.45%), and mannose (6.50%). The treatment of an experimental pleurisy
model in rats through subcutaneous administration of 50, 100, 200, and 400 mg/kg of KEPS
decreased both the volume of inflammatory exudate and the number of leukocytes recruited to
the pleural cavity. The present data showed that EPS production by K. oxytoca using the method
described is easy to perform and results in a good yield. In addition, we show that KEPS exhibit
anti-inflammatory activity when administered subcutaneously in rats
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Grant numbers 15195
