Optimization of glycerol-based medium composition for antifungal metabolites production by Bacillus subtilis
DOI:
https://doi.org/10.1590/s2175-97902022e19736%20Palavras-chave:
Bacillus subtilis, Antifungal metabolites, Neurospora crassa, Cultivation medium composition, Response surface methodologyResumo
Bacillus species are promising producers of various compounds that have pronounced antimicrobial, antiviral and antitumor activities. Due to its GRAS status, Bacillus subtilis represents an excellent candidate for the usage in plant pathogens biocontrol. In this research, evaluation of antifungal metabolites biosynthesis by Bacillus subtilis ATCC 6633 and optimization of glycerol-based medium composition, using response surface methodology, for the production of compounds effective against Neurospora crassa were investigated. The results of disc-diffusion method indicate that applied Bacillus strain produces compounds with antifungal activity against tested fungus. In order to find optimal cultivation medium composition, the experiments were carried out in accordance with Box-Behnken design, and optimization was performed using the concept of desirability function combined with previously defined mathematical equation, which describes examined bioprocess. The optimization model predicts that maximum inhibition zone diameter against Neurospora crassa of 32.24 mm is achieved when initial content of glycerol, NaNO2 and K2HPO4 were 49.68 g/L, 2.90 g/L and 6.49 g/L, respectively. Additionally, the second optimization set was made to minimize the consumption of medium components and costs of medium preparation. The obtained results are the basis for further research aimed to develop medium appropriate for economically justified production of bioactive compounds at industrial scale.
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Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45(4):493-496.
Brandão LV, Assis DJ, López JA, Espiridião MCA, Echevarria EM, Druzian JI. Bioconversion from crude glycerin by Xanthomonas campestris 2103: xanthan production and characterization. Braz J Chem Eng. 2013;30(4):737-746.
Caulier S, Nannan C, Gillis A, Licciardi F, Bragard C, Mahillon J. Overview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group. Front Microbiol. 2019;10:302.
Cladera-Olivera F, Caron GR, Brandelli A. Bacteriocin production by Bacillus licheniformis strain P40 in cheese whey using response surface methodology. Biochem Eng J. 2004;21(1):53-58.
da Silva GP, Mack M, Contiero J. Glycerol: A promising and abundant carbon source for industrial microbiology. Biotechnol Adv. 2009;27(1):30-39.
Deepak V, Kalishwaralal K, Ramkumarpandian S, Babu SV, Senthilkumar SR, Sangiliyandi G. Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology. Bioresour Technol. 2008;99(17):8170-8174.
de Faria AF, Teodoro-Martinez DS, de Oliveira Barbosa GN, Vaz BG, Silva ÍS, Garcia JS. Production and structural characterization of surfactin (C14/Leu7) produced by Bacillus subtilis isolate LSFM-05 grown on raw glycerol from the biodiesel industry. Process Biochem. 2011;46(10):1951-1957.
Demoling F, Figueroa D, Bååth E. Comparison of factors limiting bacterial growth in different soils. Soil Biol Biochem. 2007;39(10):2485-2495.
de Sousa M, Dantas IT, Felix AKN, de Sant'Ana HB, Melo VMM, Gonçalves LRB. Crude glycerol from biodiesel industry as substrate for biosurfactant production by Bacillus subtilis ATCC 6633. Braz Arch Biol Technol. 2014;57(2):295-301.
El-Banna NM. Effect of carbon source on the antimicrobial activity of the air flora. World J Microbiol Biotechnol. 2005;21:1451-1454.
El-Banna NM, Quddoumi SS. Effect of nitrogen source on the antimicrobial activity of the bacilli air flora. Ann Microbiol. 2007;57(4):669-671.
El-Banna NM, Quddoumi SS. Antimicrobial activity of Bacillus cereus: Isolation, identification and the effect of carbon and nitrogen source on its antagonistic activity. J Microbiol Antimicrob. 2016;8(2):7-13.
El-Mounadi K, Islam KT, Hernández-Ortiz P, Read ND, Shah DM. Antifungal mechanisms of a plant defensin MtDef4 are not conserved between the ascomycete fungi Neurospora crassa and Fusarium graminearum. Mol Microbiol. 2016;100(3):542-559.
El-Sersy NA, Ebrahim HAH, Abou-Elela GM. Response surface methodology as a tool for optimizing the production of antimicrobial agents from Bacillus licheniformis SN2. Curr Res Bacteriol. 2010;3(1):1-14.
Fernandes PAV, de Arruda IR, dos Santos AFAB, de Araújo AA, Maior AMS, Ximenes EA. Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant bacteria. Braz J Microbiol. 2007;38:704-709.
Ferreira SLC, Bruns RE, Ferreira HS, Matos GD, David JM, Brandão GC, et al. Box-Behnken design: An alternative for the optimization of analytical methods. Anal Chim Acta. 2007;597(2):179-186.
Grahovac J, Grahovac M, Dodić J, Bajić B, Balaž J. Optimization of cultivation medium for enhanced production of antifungal metabolites by Streptomyces hygroscopicus. Crop Prot. 2014;65:143-152.
Jacobson DJ, Dettman JR, Adams RI, Boesl C, Sultana S, Roenneberg T, et al. New findings of Neurospora in Europe and comparisons of diversity in temperate climates on continental scales. Mycologia. 2006;98(4):550-559.
Khan AAH, Naseem, Rupa L, Prathibha B. Screening and potency evaluation of antifungal from soil isolates of Bacillus subtilis on selected fungi. Adv Biotech. 2011;10(7):35-37.
Kim HS, Park J, Choi SW, Choi KH, Lee GP, Ban SJ, et al. Isolation and characterization of Bacillus strains for biological control. J Microbiol. 2003;41(3):196-201.
Koch L, Lodin A, Herold I, Ilan M, Carmeli S, Yarden O. Sensitivity of Neurospora crassa to a marine-derived Aspergillus tubingensis anhydride exhibiting antifungal activity that is mediated by the MAS1 protein. Mar Drugs. 2014;12(9):4713-4731.
Konstantinović S, Danilović B, Ćirić J, Ilić S., Savić D, Veljković V. Valorization of crude glycerol from biodiesel production. Chem Ind Chem Eng Q. 2016;22(4):461-489.
Kumar A, Saini P, Shrivastava JN. Production of peptide antifungal antibiotic and biocontrol activity of Bacillus subtilis. Indian J Exp Biol. 2009;47(1):57-62.
Lang WK, Glassey K, Archibald AR. Influence of phosphate supply on teichoic acid and teichuronic acid content of Bacillus subtilis cell walls. J Bacteriol. 1982;151(100):367-375.
Leelasuphakul W, Hemmanee P, Chuenchitt S. Growth inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit. Postharvest Biol Technol. 2008;48:113-121.
Liu S. How Cells Grow. In: Bioprocess Engineering: Kinetics, Sustainability, and Reactor Design. 2nd Edition. Amsterdam. Elsevier; 2013. p. 629-699.
Meneses EA, Durango DL, García CM. antifungal activity against postharvest fungi by extracts from colombian propolis. Quim Nova. 2009;32(8):2011-2017.
Moro IJ, Gondo GDGA, Pierri EG, Pietro RCLR, Soares CP, de Sousa DP, et al. Evaluation of antimicrobial, cytotoxic and chemopreventive activities of carvone and its derivatives. Braz J Pharm Sci. 2017;53(4):e00076.
Mwakinyali SE, Ding X, Ming Z, Tong W, Zhang Q, Li P. Recent development of aflatoxin contamination biocontrol in agricultural products. Biol Control. 2019;128:31-39.
Radovanović N, Milutinović M, Mihajlovski K, Jović J, Nastasijević B, Rajilić-Stojanović M, et al. Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. Microb Pathog. 2018;120:71-78.
Rajaofera MJN, Jin PF, Fan YM, Sun QQ, Huang WK, Wang WB, et al. Antifungal activity of the bioactive substance from Bacillus atrophaeus strain HAB-5 and its toxicity assessment on Danio rerio. Pestic Biochem Physiol. 2018;147:153-161.
Thompson LJ, Gray V, Lindsay D, von Holy A. Carbon : nitrogen : phosphorus ratios influence biofilm formation by Enterobacter cloacae and Citrobacter freundii. J Appl Microbiol. 2006;101(5):1105-1113.
Turner BC, Perkins DD, Fairfield A. Neurospora from natural populations: A global study. Fungal Genet Biol. 2001;32(2):67-92.
Veličković TD, Rončević ZZ, Grahovac JA, Jokić AI, Mitrović IŽ, Bajić BŽ, et al. Formulation of medium for bactericide biosynthesis using wild type Streptomyces sp. Chem Ind Chem Eng Q . 2018;24(4):399−410.
Wang ZW, Liu XL. Medium optimization for antifungal active substances production from a newly isolated Paenibacillus sp. using response surface methodology. Bioresour Technol . 2008;99(17):8245-8251.
Wise C, Novitsky L, Tsopmo A, Avis TJ. Production and antimicrobial activity of 3-hydroxypropionaldehyde from Bacillus subtilis strain CU12. J Chem Ecol. 2012;38:1521-1527.
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