Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis: biodistribution on 4T1 tumor-bearing mice

Nicole Valerie Lecot; Gonzalo Rodríguez; Valentina  Stancov; Marcelo Fernández; Mercedes González; Romina Glisoni; Pablo Cabral; Hugo Cerecetto

doi:10.1590/s2175-97902022e191055

Autores

Nicole Valerie Lecot Laboratorio de Técnicas Nucleares Aplicadas a Bioquímica y Biotecnología, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Uruguay
Gonzalo Rodríguez Grupo de Química Orgánica Medicinal, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Uruguay
Valentina Stancov Grupo de Química Orgánica Medicinal, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Uruguay
Marcelo Fernández Laboratorio de Experimentación Animal, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Uruguay
Mercedes González Grupo de Química Orgánica Medicinal, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Uruguay
Romina Glisoni Departamento de Tecnología Farmacéutica, Cátedra de Tecnología Farmacéutica II, CONICET-Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
Pablo Cabral Área de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Uruguay
Hugo Cerecetto Grupo de Química Orgánica Medicinal, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Uruguay; Área de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Uruguay https://orcid.org/0000-0003-1256-3786

DOI:

https://doi.org/10.1590/s2175-97902022e191055

Palavras-chave:

Amphiphilic polymeric micelles, T1307, BODIPY, 99mTc, In vivo biodistribution, Cancer diagnosis

Resumo

In recent years, nanocarriers have been studied as promising pharmaceutical tools for controlled drug-delivery, treatment-efficacy follow-up and disease imaging. Among them, X-shaped amphiphilic polymeric micelles (Tetronic®, poloxamines) display great potential due to their biocompatibility and non-toxic effects, among others. In the present work, polymeric micelles based on the T1307 copolymer were initially decorated with a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)-fluorophore in order to determinate its in vivo biodistribution on 4T1 tumor-bearing mice. However, unfavorable results with this probe led to two different strategies. On the one hand, the BODIPY-micelle-loaded, L-T1307-BODIPY, and on the other hand, the ^99mTc-micelle-radiolabeled, L-T1307- ^99m Tc, were analyzed separately in vivo. The results indicated that T1307 accumulates mainly in the stomach, the kidneys, the lungs and the tumor, reaching the maximum organ-accumulation 2 hours after intravenous injection. Additionally, and according to the results obtained for L-T1307- ^99m Tc, the capture of the polymeric micelles in organs could be observed up to 24 hours after injection. The results obtained in this work were promising towards the development of new radiotracer agents for breast cancer based on X-shaped polymeric micelles.

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Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis

biodistribution on 4T1 tumor-bearing mice

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