Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications

Part II: Stability, Cytotoxicity and Silver Release Patterns

Autores

  • Lillian Vieira Vaz PhageLab- Laboratory of Biofilms and Bacteriophages, University of Sorocaba Campus Cidade Universitária Prof. Aldo Vannucchi, Sorocaba/SP, Brazil
  • Victor Manuel Balcão PhageLab- Laboratory of Biofilms and Bacteriophages, University of Sorocaba Campus Cidade Universitária Prof. Aldo Vannucchi, Sorocaba/SP, Brazil; Department of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal
  • José Martins Oliveira Junior PhageLab- Laboratory of Biofilms and Bacteriophages, University of Sorocaba Campus Cidade Universitária Prof. Aldo Vannucchi, Sorocaba/SP, Brazil
  • Matthieu Tubino Institute of Chemistry, University of Campinas, Campinas/SP, Brazil
  • Angela Jozala PhageLab- Laboratory of Biofilms and Bacteriophages, University of Sorocaba Campus Cidade Universitária Prof. Aldo Vannucchi, Sorocaba/SP, Brazil
  • Valquiria Miwa Hanai Yoshida PhageLab- Laboratory of Biofilms and Bacteriophages, University of Sorocaba Campus Cidade Universitária Prof. Aldo Vannucchi, Sorocaba/SP, Brazil
  • Marta Maria Duarte Carvalho Vila PhageLab- Laboratory of Biofilms and Bacteriophages, University of Sorocaba Campus Cidade Universitária Prof. Aldo Vannucchi, Sorocaba/SP, Brazil https://orcid.org/0000-0002-0198-7076

DOI:

https://doi.org/10.1590/s2175-97902022e18688

Palavras-chave:

Hydrogel, Silver sulfadiazine, Antimicrobial topical applications

Resumo

Hydrogels are interesting for use in the treatment of topical wounds due to their virtually zero toxicity, and capacity for extended release of pharmaceuticals. Silver sulfadiazine (SSDZ) is the drug of choice in the treatment of skin burns. The aim of the study was to determine cytotoxicity, antimicrobial activity and stability of a PVA hydrogel with integrated silver sulfadiazine. SSDZ-hydrogels were prepared using 10% (w/w) PVA (either 89% or 99% hydrolyzed) and 1% (w/w) silver sulfadiazine. Cellular viability was assessed via MTS assays, antimicrobial activity via disk-diffusion and accelerated stability tests were carried out with analysis at 0, 30, 60, 90 and 180 days of storage at 40 ± 2 °C and a relative humidity of 75 ± 5%. The parameters evaluated included organoleptic characteristics, moisture, swelling ability, mechanical strength, FTIR, XRD, TGA and DSC, and silver release patterns via XRD and potentiometry. Cell viability tests indicated some cytotoxicity, although within acceptable levels. After 90 days of storage, SSDZ hydrogel samples exhibited a brown coloration, probably due to the formation of Ag or Ag2O nanoparticles. The SSDZ-loaded hydrogels suffered visual and physical changes; however, these changes did not compromise its use as occlusive wound dressings or its antimicrobial properties.

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Publicado

2022-11-10

Edição

v. 58 (2022)

Seção

Original Article

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Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences

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Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications: Part II: Stability, Cytotoxicity and Silver Release Patterns. (2022). Brazilian Journal of Pharmaceutical Sciences, 58. https://doi.org/10.1590/s2175-97902022e18688

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