Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples
DOI:
https://doi.org/10.1590/s2175-97902022e19604%20Keywords:
Avermectins, Milbemycins, Quality control, Bulk samplesAbstract
This work aims to develop analytical methods using high-performance liquid chromatography
with a diode array detector (HPLC-DAD) for analysis and quantification of avermectins
(AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized and
some parameters such as temperature, flow rate, injection volume and mobile phase with
different proportions of solvents were evaluated. The best chromatographic conditions were
obtained using the Phenomenex® C18 (150 × 4.60 mm, 5 μm) column at a temperature of 20
°C, flow rate of 1.2 mL min–1, injection volume of 20 μL, and detection at 250 nm. Acetonitrile:
ultrapure water (87: 13, v/v) was used as mobile phase for moxidectin and eprinomectin, and
acetonitrile: methanol: ultrapure water (53: 35: 12, v/v/v) for abamectin and ivermectin. Under
these conditions satisfactory results were obtained, with appropriate limits of detection and
quantification, acceptable linearity, precision, accuracy, and robustness. These methods satisfy
the need for analytical methods for the multi-determination of MBMs and the B1a and B1b
forms of AVMs by HPLC-DAD, which can be considered simple, effective, innovative and
should aid in the development of the field.
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