Chemical composition and biological activity of Baccharis erioclada DC. essential oil
DOI:
https://doi.org/10.1590/s2175-97902022e19118%20Keywords:
Volatile oil, Asteraceae, Vassoura-lageanaAbstract
The chemically complex essential oils of Baccharis species are associated with several biological activities, such as antimicrobial and antiulcerous properties. However, few studies have investigated Baccharis erioclada DC. Therefore, in this study, we aimed to characterize the essential oil of B. erioclada and evaluate its antioxidant, antimicrobial, and hemolytic potential. The essential oil was extracted by hydrodistillation using a Clevenger apparatus and analyzed via gas chromatography-mass spectrometry (GC-MS). Phosphomolybdenum complex formation, reducing antioxidant power, and thiobarbituric acid reactive substances (TBARS) methods were used to determine antioxidant potential. To evaluate the essential oil’s antimicrobial activity, minimum inhibitory concentrations (MIC) in Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans were calculated. Hemolytic activity was determined in sheep red blood cells. Thirty-one compounds were identified via GC-MS analysis, representing 81.60% of the total essential oil. These compounds included: turmerone (27.97%), fokienol (13.47%), ledol (9.78%), and santalol (5.35%). The class of compounds identified was the oxygenated sesquiterpenes (62.52%). Antioxidant activity was confirmed via phosphomolybdenum complex formation and TBARS methods. Moderate antimicrobial activity and low hemolysis rates were displayed at concentrations of 250 and 500 μg/mL.
Downloads
References
Abad MJ, Bermejo P. Baccharis (Compositae): a review update. Caldasia. 2007;7:76-96.
Adams RP. Identification of essential oils components by gas chromatography mass spectroscopy. 4.ed. Illinois: Allured Publishing Corporation. Carol Stream. 2007.
Agostini F, Santos ACA, Rossato M, Pansera MR, Zattera F, Wasum R, et al. Estudo do óleo essencial de algumas espécies do gênero Baccharis (Asteraceae) do sul do Brasil. Rev Bras Farmacogn. 2005;15(3):215-220.
Ajaiyeoba EO, Sama W, Essien EE, Olayemi JO, Ekundayo O, Walker TM, et al. Larvicidal activity of turmerone-rich essential oils of Curcuma longa leaf and rhizome from Nigeria on Anopheles gambiae. Pharm Biol. 2008;46(4):279-282.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oil: a review. Food Chem Toxicol. 2008;46(2):446-75.
Banerjee A, Kunwar A, Mishra B, Priyadarsini KI. Concentration dependent antioxidant/pro-oxidant activity of curcumin studies from AAPH induced hemolysis of red blood cells. Chem-Biol Interact. 2008;174(2):134-139.
Barbosa LCA, Filomeno CA, Teixeira RR. Chemical variability and biological activities of Eucalyptus spp. volatile oils. Molecules. 2016;21(12):1671.
Bednarczuk VO, Verdam MCS, Miguel MD, Miguel OG. Testes in vitro e in vivo utilizados na triagem toxicológica de produtos naturais. Visão acadêmica. 2010;11(2):43-50.
Bobek VB, Almeida VP, Pereira CB, Heiden G, Duarte, MR, Budel JM, et al. Comparative pharmacobotanical analysis of Baccharis caprariifolia DC. and B. erioclada DC. from Campos Gerais, Paraná, Southern Brazil. Lat Am J Pharm. 2015;34(7):1396-402.
Bogo CA, Andrade MH, Paula JP, Farago PV, Doll-Boscardin PM, Budel JM. Comparative analysis of essential oils of Baccharis L.: a review. Rev Stricto Sensu. 2016;1(2):1-11.
Boix YF, Victório CP, Lage CLS, Kuster RM. Volatile compounds from Rosmarinus officinalis L. and Baccharis dracunculifolia DC. growing in southeast coast of Brazil. Quim Nova. 2010;33(2):255-257.
BRASIL. Ministério da Saúde. Secretaria de Vigilância Sanitária. Portaria n. 116, de 8.8.1996. Diário Oficial da República Federativa do Brasil, 12 ago 1996.
Brooker MIH, Kleinig DA. Field guide to Eucalyptus (3rd ed.), Vol. 1. Melbourne: Bloomings; 2006.
Budel JM, Duarte MR, Doll-Boscardin PM, Farago PV, Matzenbacher NI, Sartoratto A, et al. Composition of essential oils and secretory structures of Baccharis anomala, B. megapotamica and B. ochracea. J Essent Oil Res. 2012;24(1):19-24.
Budel JM, Raman V, Monteiro LM, Almeida VP de, Bobek VB, Heiden G, et al. Foliar anatomy and microscopy of six Brazilian species of Baccharis (Asteraceae). Microsc Res Tech. 2018;81(8):832-842.
Búfalo MC, Candeias JM, Sousa JP, Bastos JK, Sforcin JM. In vitro cytotoxic activity of Baccharis dracunculifolia and propolis against HEp-2 cells. Nat Prod Res. 2010;24(18):1710-1718.
Campos FR, Bressan J, Jasinski VCG, Zuccolotto T, Silva LE, Cerqueira, LB. Baccharis (Asteraceae): Chemical constituents and biological activities. Chem Biodivers. 2016;13(1):1-17.
Chaaban A, Martins CEN, Bretanha LC, Micke GA, Carrer AR, Ferreira L, et al. Insecticide activity of Baccharis dracunculifolia essential oil against Cochliomyia macellaria (Diptera: Calliphoridae). Nat Prod Res . 2017;32(24):2954-2958.
Duncan DB. Multiple range and multiple F tests. Biometrics. 1955;11:1-42.
Fabiane KC, Ferronatto R, Santos AC, Onofre SB. Physicochemical characteristics of the essential oils of Baccharis dracunculifolia and Baccharis uncinella DC. (Asteraceae). Rev Bras Farmacogn . 2008;18(2):197-203.
Ferracini VL, Paraiba LC, Leitão-Filho HF, Silva AG, Nascimento LR, Marsaioli AJ. Essential oils of seven Brazilian Baccharis species. J Essen Oil Res. 1995;7(4):355-367.
Ferreira FD, Kemmelmeier C, Arrotéia CC, da Costa CL, Mallmann CA, Janeiro V, et al. Inhibitory effect of the essential oil of Curcuma longa L. and curcumin on aflatoxin production by Aspergillus flavus Link. Food Chem. 2013;136(2):789-793.
Ferronatto R, Marchesan ED, Pezenti E, Bednarski F, Onofre SB. Atividade antimicrobiana de óleos essenciais produzidos por Baccharis dracunculifolia DC. e Baccharis uncinella DC. (Asteraceae). Rev Bras Farmacogn . 2007;17(2):224-230.
Flora do Brasil 2020 em construção. Jardim Botânico do Rio de Janeiro. [Cited 2018 June 6]. Available from: Available from: http://floradobrasil.jbrj.gov.br
» http://floradobrasil.jbrj.gov.br
Florão FA, Budel JM, Duarte MR, Marcondes A, Rodrigues RAF, Rodrigues MVN, et al. Essential oil from Baccharis species (Asteraceae) have anti-inflammatory effects for human cells. J Essent Oil Res. 2012;24(6):561-570.
Galindo LA, Pultrini AM, Costa M. Biological effects of Ocimum gratissimum L. are due to synergic action among multiple compounds present in essential oil. J Nat Med. 2010;64(4):436-441.
Heinzmann BM, Spitzer V, Simões CMO. Oléos essenciais. In: Simões CMO, Schenkel EP, Mello JCP, Mentz LA, Petrovick PR, editors. Farmacognosia: do produto natural ao medicamento. Porto Alegre: Artmed; 2017.
Jayanthi P, Lalitha P. Reducing power of the solvent extracts of Eichhorniacrassipes (mart.) Solms. Int J Pharm Pharm Sci. 2011;3(3):126-128.
Kurdelas RR, Kurdelasa RR, Sandra López S, Lima B, Feresin GE, Zygadloc J, et al. Chemical composition, anti-insect and antimicrobial activity of Baccharis darwinii essential oil from Argentina, Patagonia. Ind Crops Prod. 2012;40:261-267.
Lago JHG, Romoff P, Fávero AO, Soares MG, Baraldi PT, Corrêa AG, et al. Composição química dos óleos essenciais das folhas de seis espécies do gênero Baccharis de “Campos de Altitude” da mata atlântica paulista. Quím Nova. 2008;31(4):727-730.
Lemos DRH, Melo EC, Rocha RP, Barbosa LCA, Pinheiro AL. Influence of drying air temperature on the chemical composition of the volatile oil of melaleuca. Eng Agric. 2012;20(1):5-11.
Li X, Wang W, Luo M. Solvent-free microwave extraction of essential oil from Dryopteris fragrans and evaluation of antioxidant activity. Food Chem . 2012;133(2):427-444.
Lima MRF, Luna JS, Santos AF. Andrade, MCC, San’t Ana, AEG, Genet, JP.Anti-bacterial activity of some Brazilian medicinal plants. J Ethnopharmacol. 2006;105:137-14.
Maciel MAM, Pinto AC, Veiga JRVV, Grynberg NF, Echvarria A. Plantas medicinais: A necessidade de estudos multidisciplinares. Quím Nova . 2002;25(3):429-438.
Massignani JJ, Lemos M, Maistro EL, Schaphauser HP, Jorge RF, Sousa JP, et al. Antiulcerogenic activity of the essential oil of Baccharis dracunculifolia on different experimental models in rats. Phytother Res. 2009;23:1355-60.
Miguel, M. G. Antioxidant and anti-inflammatory activities of essential oils: a short review. Molecules . 2010;15(12):9252-9287.
Morais SM, Catunda Júnior FEA, Silva ARA, Martins Neto JS. Atividade antioxidante de óleos essenciais de espécies de Croton do nordeste do Brasil. Quim Nova . 2006;29(5):907-10.
Negreiros MO, Pawlowski A, Zini CA, Soares GL, Motta AS, Frazzon AP. Antimicrobial and antibiofilm activity of Baccharis psiadioides essential oil against antibiotic-resistant Enterococcus faecalis strains. Pharm Biol. 2016;54(12):3272-3279.
Parreira NA, Magalhães LG, Morais, DR, Caixeta, SC, Sousa JP, Bastos JK, et al. Antiprotozoal, schistosomicidal, and antimicrobial activities of the essential oil from the leaves of Baccharis dracunculifolia. Chem Biodivers . 2010;7(4):993-1001.
Pereira CB, Farago PV, Borsato DM, Folquitto DG, Maia BHLNS, Esmerino LA, et al. Chemical composition and biological activities of Baccharis milleflora essential oil. Lat. Am J Pharm. 2016;35(10):2225-2233.
Pereira CB, Kanunfre CC, Farago PV, Borsato DM, Budel JM, de Noronha Sales Maia BHL, et al. Cytotoxic mechanism of Baccharis milleflora (Less.) DC. essential oil. Toxicol In Vitro. 2017;42:214-221.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269:337-341.
Santos LMO, Oliveira LA, Tibulo EPS, Lima CP. Análise de amostras de flores de Calêndula (Calendula officinalis L., Asteraceae) comercializadas na grande Curitiba. Rev Ciênc Farm Básica Apl. 2015;36:251-258.
Santos SC, Ferreira FS, Rossi-Alva JC, Fernandez LG. Atividade antimicrobiana in vitro do extrato de Abarema cochliocarpos (Gomes) Barneby & Grimes. Rev Bras Farmacogn . 2007;17(2):215-219.
Sonaglio D, González Ortega G, Petrovick PR, Bassani VL. Farmacognosia - da planta ao medicamento. Capítulo 13: Desenvolvimento Tecnológico e Produção de Fitoterápicos.6. ed. Editora da UFRGS. Editora da UFSC. 2007.
The United States Pharmacopeia. 25th ed. Rockville: United States Pharmacopeial Convention; 2002.
Vannini AB, Santos TG, Fleming LRP, Purnhagen LA, Lourenço ETB, Butzke M, et al. Chemical characterization and antimicrobial evaluation of the essential oils from Baccharis uncinella DC. and Baccharis semiserrata DC. (Asteraceae). J Essent Oil Res. 2012;24(6):547-554.
Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem . 1995;43(1):27-32.
Zapata B, Duran C, Stashenko E, Betancur-Galvis L, Mesa-Arango AC. Actividad antimicótica y citotóxica de aceites esenciales de plantas de la familia Asteraceae. Rev Iberoam Micol. 2010;27(2):101-103.
Zohra M, Fawzia A. Hemolytic activity of different herbal extracts used in Algeria. Int J Pharma Sci Res. 2014;5(8):495-500.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.
The on line journal has open and free access.
