In vitro anti-inflammatory, anti-arthritic and antiproliferative activity of green synthesized silver nanoparticles - Phoenix dactylifera (Rothan dates)
DOI:
https://doi.org/10.1590/s2175-97902022e18594%20Keywords:
Nanoparticles, Proliferation, Inflammatory, Arthritic, Green synthesis, AnticancerAbstract
Traditionally dates is consumed as a rich source of iron supplement and the current research discuss the synthesis of silver nanoparticles (AgNPs) using methanolic seed extract of Rothan date and its application over in vitro anti-arthritic, anti-inflammatory and antiproliferative activity against lung cancer cell line (A549). FTIR result of synthesised AgNPs reveals the presence of functional group OH as capping agent. XRD pattern confirms the crystalline nature of the AgNPs with peaks at 38º, 44º, 64º and 81º, indexed by (111), (200), (220) and (222) in the 2θ range of 10-90, indicating the face centered cubic (fcc) structure of metallic Ag. HR- TEM results confirm the morphology of AgNPs as almost spherical with high surface areas and average size of 42 ± 9nm. EDX spectra confirmed that Ag is only the major element present and the Dynamic light scattering (DLS) assisted that the Z-average size was 203nm and 1.0 of PdI value. Zeta potential showed − 26.5mv with a single peak. The results of the biological activities of AgNPs exhibited dose dependent activity with 68.44% for arthritic, antiinflammatory with 63.32% inhibition and anti-proliferative activity illustrated IC50 value of 59.66 μg/mL expressing the potential of AgNPs to combat cancer.
Downloads
References
Ahmed S, Saifullah Ahmad M, Swami BL, Ikram S. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J Radiat Res Appl Sci. 2016;6:42-47. doi.org/10.1016/j.jrras.2015.06.006
» https://doi.org/10.1016/j.jrras.2015.06.006
Arunachalam T, Khader SZA, Syed Zameer Ahmed S, Vetrivel M, SyedAmeen S, Ameer Khadharu IS, et al. Radical scavenging and antiproliferative effect of novel phenolic derivatives isolated from Nerium indicum against human breast cancer cell line (MCF-7)-an in silico and in vitro approach. Environ Sci Pollut Res. 2020;27(9):9038-9057. doi.org/10.1007/s11356-019-07252-x
» https://doi.org/10.1007/s11356-019-07252-x
Baghizadeh A, Ranjbar S, Gupta VK, Asif M, Pourseyedi S, Karimi MJ, et al. Green synthesis of silver nanoparticles using seed extract of Calendula officinalis in liquid phase. J Mol Liq. 2015;207:159-163. doi.org/10.1016/j.molliq.2015.03.029
» https://doi.org/10.1016/j.molliq.2015.03.029
Banerjee P, Satapathy M, Mukhopahayay A, Das P. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour, Bioprocess. 2014;1(3):1-10. doi.org/10.1186/s40643-014- 0003-y
» https://doi.org/10.1186/s40643-014- 0003-y
Deshpande V, Jadhav VM, Kadam VJ. In vitro anti-arthritic activity of Abutilon indicum (Linn.) Sweet. J Pharm Res. 2009;2(4):644-645.
Dhand V, Soumya L, Bharadwaj S, Chakra S, Bhatt D, Sreedhar B. Green Synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Mater Sci Eng. 2016;58:36-43. doi.org/10.1016/j.msec.2015.08.018
» https://doi.org/10.1016/j.msec.2015.08.018
Farhadi S, Ajerloo B, Mohammadi A. Green biosynthesis of spherical Silver nanoparticles by using Date Palm (Phoenix Dactylifera) fruit extract and study of their antibacterial and catalytic activities. Acta Chim Slov. 2017;64(1):129-143. doi.10.17344/acsi.2016.2956
» https://doi.org/10.17344/acsi.2016.2956
Haghighi Pak Z, Abbaspour H, Naser Karimi N, Fattahi A. Eco-Friendly Synthesis and Antimicrobial Activity of Silver Nanoparticles Using Dracocephalum moldavica Seed Extract. Appl Sci. 2016;6:69. doi.org/10.3390/app6030069
» https://doi.org/10.3390/app6030069
Halliwell B, John MCGutteridge, Aruoma OI. Thedeoxyribose method: a simple test tube assay for the determination of rate constants for reactions of hydroxyl radicals. Anal Biochem. 1987;165(1):215-219. doi.org/10.1016/0003-2697(87)90222-3
» https://doi.org/10.1016/0003-2697(87)90222-3
Jayaseelana C, Ramkumar R, Rahumana AA, Perumal P. Green synthesis of gold nanoparticles using seed aqueous extract of Abelmoschus esculentus and its antifungal activity. Ind Crops Prod. 2013;45:423-429. doi.org/10.1016/j. indcrop.2012.12.019
» https://doi.org/10.1016/j. indcrop.2012.12.019
Kehili HE, Zerizer S, Beladjila KA, Kabouche Z. Anti-inflammatory effect of Algerian date fruit (Phoenix dactylifera). Food Agric Immunol. 2016;27(6):820-829. doi.org/10.1080/09540105.2016.1183597
» https://doi.org/10.1080/09540105.2016.1183597
Khader SZA, Syed Zameer Ahmed S, Ganesan GM, Mahboob MR, Vetrivel M, Sankarappan M, et al. Rhynchosia rufescens AgNPs enhance cytotoxicity by ROS-mediated apoptosis in MCF-7 cell lines. Environ Sci Pollut Res . 2020;27:2155-2164. doi.org/10.1007/s11356-019-06479-y
» https://doi.org/10.1007/s11356-019-06479-y
Khader SZA, Syed Zameer Ahmed S, Sathyan J, Mahboob MR, Venkatesh KP, Ramesh K. A comparative study on larvicidal potential of selected medicinal plants over green synthesized silver nano particles. Egypt J Basic Appl Sci. 2018a;5(1):54-62. doi.org/10.1016/j.ejbas.2018.01.002
» https://doi.org/10.1016/j.ejbas.2018.01.002
Khader SZA, Syed Zameer Ahmed S, Arunachalamb T, Sanjeeva N, Balasubramanian SK, SyedAmeen ST, et al. Radical scavenging potential, antiinflammatory and antiarthritic activity of isolated Isomer Methyl-γ-Orsellinate and Roccellatol from Roccella Montagnei. Bulletin of Faculty of Pharmacy, Cairo University, 2018b;56(1):39-45. doi.org/10.1016/j.bfopcu.2018.02.001
» https://doi.org/10.1016/j.bfopcu.2018.02.001
Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol. 1968;20(3):169-173. doi.org/10.1111/j.2042-7158.1968.tb09718.x
» https://doi.org/10.1111/j.2042-7158.1968.tb09718.x
Oyaizu M. Studies on products of browning reactions prepared from glucosamine. Japanese J Nutrition. 1986;44(6):307-315. doi.org/10.5264/eiyogakuzashi.44.307
» https://doi.org/10.5264/eiyogakuzashi.44.307
Packialakshmi N, Suganya C, Guru V. Antibacterial activity and green synthesis of silver nanoparticles using Strychnos potatorum seed and bark extract. Asian J Phytomed Clin Res. 2014;2(3):127-138.
Rafi MM, Ahmed KSZ, Nazeer KP, Kumar SD, Thamilselvan M. Synthesis, characterization and magnetic properties of hematite (α-Fe2O3) nanoparticles on polysaccharide templates and their antibacterial activity. Appl Nanosci. 2015;5:515-520. doi.org/10.1007/s13204-014-0344-z
» https://doi.org/10.1007/s13204-014-0344-z
Rosarin FS, Arulmozhi V, Nagarajan S, Mirunalini S. Antiproliferative effect of silver nanoparticles synthesized using amla on Hep2 cell line. Asian Pac J Trop Med. 2013;6(1):1-10. doi.org/10.1016/S1995-7645(12)60193-X
» https://doi.org/10.1016/S1995-7645(12)60193-X
Roy A, Bulut O, Some S, Mandal AK, Yilmaz DM. Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity. RSC Adv. 2019;9:2673-2702. doi.org/10.1039/C8RA08982E
» https://doi.org/10.1039/C8RA08982E
Shankar SS, Rai A, Amad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core - Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci. 2004;275(2):496-502. doi.org/10.1016/j.jcis.2004.03.003
» https://doi.org/10.1016/j.jcis.2004.03.003
Shib SD, Arun KS, Braja GB, Sujoy B. Phoenix dactylifera (Date Palm) Seed Extract Mediated Green Synthesis of Gold Nanoparticles and its Application as a Catalyst for the Reduction of 4-nitrophenol to 4-aminophenol. Int J Nanomat Biostru. 2013;2(3):42-46.
Shimada K, Fujikawa KK, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem. 1992;40(6):945-948. doi. 10.1021/jf00018a005
» https://doi.org/10.1021/jf00018a005
Shravan Kumar N, Kishore G, Siva Kumar G, Sindhu Priya ES. In vitro anti-inflammatory and anti-arthritic activity of leaves of Physalis angulata L. Int J Pharm Ind Res. 2011;1(3):211-213.
Sidhra S, Syed Zameer Ahmed K, Krishnaveni R, Anupriya B, Senthil Kumar B, Kishore R. Modulatory effect of Leucas aspera on oxidative stress and glucose metabolism against diabetic complications in experimental rats. Int Res J Pharm. 2017;8(8):27-33. doi.10.7897/2230-8407.088140
» https://doi.org/10.7897/2230-8407.088140
Syed Zameer Ahmed K, Sidhra S, Thangakumar A, Krishnaveni R. Therapeutic effect of Parmotrema tinctorum against complete Freund’s adjuvant-induced arthritis in rats and identification of novel Isophthalic ester derivative. Biomed Pharmacother. 2019a;112:108646. doi.org/10.1016/j.biopha.2019.108646
» https://doi.org/10.1016/j.biopha.2019.108646
Syed Zameer Ahmed S, Khader SZA, Radhakrishnana K, Marimuthua V, Chinnusamy M, Thangavel V, et al. Antiobesity and antihyperlipidemic effect of Ixora coccinea on triton-X100 induced hyperlipidemia in rats- an approach to evaluate Asymmetrical Temperature Distribution Analysis Using Thermography. Chinese Herbal Medicines. 2019b;11(3):326-331 doi.org/10.1016/j.chmed.2019.05.006
» https://doi.org/10.1016/j.chmed.2019.05.006
Venugopal K, Rather HA, Rajagopal K, Shanthi MP, Sheriff K, Illiyas M, et al. Synthesis of silver nanoparticles (AgNPs) for anticancer activities (MCF 7 breast and A549 lung cell lines) of the crude extract of Syzygium aromaticum. J Photochem Photobiol B. 2017;167:282-289. doi.org/10.1016/j.jphotobiol.2016.12.013
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.
The on line journal has open and free access.
