Comparison of the biocompatibility of grey mineral trioxide aggregate and sealapex plus zinc oxide in rat subcutaneous tissue
DOI:
https://doi.org/10.11606/issn.2357-8041.clrd.2015.109271Palavras-chave:
Biocompatibility Testing, Endodontics, Dental Materials, Retrograde Obturation.Resumo
ABSTRACT
Objectives:The aim of this study was to compare the subcutaneous tissue response to grey mineral trioxide aggregate white Sealapex plus zinc oxide. Methods: Polyethylene tubes filled with tested material were implanted in the connective tissue of rats. Control animals received empty tubes. Tissue samples were collected after 7, 60, and 90 days and stained with hematoxylin-eosin, picrosirius-fast green, and von Kossa stain for morphological analysis. The connective tissue response to the implanted materials was evaluated descriptively and semi-quantitatively by scoring the degree of inflammation, granulation tissue formation, fibrosis, and calcification. Results: Examinations of the grey mineral trioxide aggregate group over time revealed more intense inflammation at 7 days than at 60 days (p <0.05). In the Sealapex plus zinc oxide group, granulation tissue was more abundant at 7 days than at 60 days (p <0.05). Regarding calcification, von Kossa-positive granules were observed in the grey mineral trioxide aggregate and Sealapex plus zinc oxide groups at all time points studied. In the Sealapex/ZnO group, calcification was more apparent at 60 days than at 7 days (p <0.05). Relevance: This study demonstrates that all tested materials promote similar tissue reactions.
Descriptors: Biocompatibility Testing, Endodontics, Dental Materials, Retrograde Obturation.
Downloads
Referências
Casella G, Ferlito S. The use of mineral trioxide aggregate in endodontics. Minerva Stomatol 2006 ;55:123–43.
Cintra LTA, Bernabé PFE, de Moraes IG, Gomes-Filho JE, Okamoto T, Consolaro A, et al. Evaluation of subcutaneous and alveolar implantation surgical sites in the study of the biological properties of root-end filling endodontic materials. J Appl Oral Sci 2010;18:75–82.
Cintra LTA, Ribeiro TAA, Gomes-Filho JE, Bernabé PFE, Watanabe S, Facundo AC da S, et al. Biocompatibility and biomineralization assessment of a new root canal sealer and root-end filling material. Dent Traumatol 2013;29:145–50.
Cunha SA, Rached FJA, Alfredo E, León JE, Perez DE da C. Biocompatibility of sealers used in apical surgery: a histological study in rat subcutaneous tissue. Braz Dent J 2011;22:299–305.
De Morais CAH, Bernardineli N, Garcia RB, Duarte MAH, Guerisoli DMZ. Evaluation of tissue response to MTA and Portland cement with iodoform. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;102:417–21.
Duarte MA, Demarchi AC, Giaxa MH, Kuga MC, Fraga SC, de Souza LC. Evaluation of pH and calcium ion release of three root canal sealers. J Endod 2000;26:389–90.
Duarte MAH, Demarchi ACC de O, Yamashita JC, Kuga MC, Fraga S de C. pH and calcium ion release of 2 root-end filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95:345–7.
Gomes-Filho JE, Bernabé PFE, Nery MJ, Otoboni-Filho JA, Dezan-Júnior E, de Moraes Costa MMT, et al. Reaction of rat connective tissue to a new calcium hydroxide-based sealer. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;106:71–6.
Gomes-Filho JE, Watanabe S, Bernabé PFE, de Moraes Costa MT. A mineral trioxide aggregate sealer stimulated mineralization. J Endod 2009;35(2):256–60.
Gomes-Filho JE, Rodrigues G, Watanabe S, Estrada Bernabé PF, Lodi CS, Gomes AC, et al. Evaluation of the tissue reaction to fast endodontic cement (CER) and Angelus MTA. J Endod 2009;35:1377–80.
Gomes-Filho JE, Gomes AC, Watanabe S, Oliveira SHP de, Bernabé PFE, Percinoto C. Evaluation of tissue reaction, cell viability and cytokine production induced by Sealapex Plus. J Appl Oral Sci 2011;19:329–36.
Gomes-Filho JE, Watanabe S, Lodi CS, Cintra LTA, Nery MJ, Filho JAO, et al. Rat tissue reaction to MTA FILLAPEX®. Dent Traumatol 2012;28:452–6.
Holland R, de Souza V. Ability of a new calcium hydroxide root canal filling material to induce hard tissue formation. J Endod 1985;11:535–43.
Holland R, de Souza V, Nery MJ, Bernabé o FE, Filho JAO, Junior ED, et al. Calcium salts deposition in rat connective tissue after the implantation of calcium hydroxide-containing sealers. J Endod 2002;28:173–6.
Queiroz AM de, Assed S, Consolaro A, Nelson-Filho P, Leonardo MR, Silva RAB, et al. Subcutaneous connective tissue response to primary root canal filling materials. Braz Dent J 2011 ;22:203–11.
Lee SJ, Monsef M, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod. 1993;19:541–4.
Shahi S, Rahimi S, Yavari HR, Mokhtari H, Roshangar L, Abasi MM, et al. Effect of mineral trioxide aggregates and Portland cements on inflammatory cells. J Endod. 2010;36:899–903.
Silveira CMM, Pinto SCS, Zedebski R de AM, Santos FA, Pilatti GL. Biocompatibility of four root canal sealers: a histopathological evaluation in rat subcutaneous connective tissue. Braz Dent J 2011;22:21–7.
Tanomaru-Filho M, Luis MR, Leonardo MR, Tanomaru JMG, Silva LAB. Evaluation of periapical repair following retrograde filling with different root-end filling materials in dog teeth with periapical lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;102:127–32.
Tavares CO, Böttcher DE, Assmann E, Kopper PMP, de Figueiredo JAP, Grecca FS, et al. Tissue reactions to a new mineral trioxide aggregate-containing endodontic sealer. J Endod 2013;39:653–7.
Torabinejad M, Hong CU, Mc Donald F, Pitt Ford TR. Physical and chemical properties of a new root-end filling material. J Endod 1995;21:349–53.
Torabinejad M, Pitt Ford TR. Root end filling materials: a review. Endod Dent Traumatol. 1996;12:161–78.
Yaltirik M, Ozbas H, Bilgic B, Issever H. Reactions of connective tissue to mineral trioxide aggregate and amalgam. J Endod 2004;30:95–9.
Zmener O, Martinez Lalis R, Pameijer CH, Chaves C, Kokubu G, Grana D. Reaction of rat subcutaneous connective tissue to a mineral trioxide aggregate-based and a zinc oxide and eugenol sealer. J Endod 2012;38:1233–8.
Downloads
Publicado
Edição
Seção
Licença
Solicita-se aos autores enviar, junto com a carta aos Editores, um termo de responsabilidade. Dessa forma, os trabalhos submetidos à apreciação para publicação deverão ser acompanhados de documento de transferência de direitos autorais, contendo a assinatura de cada um dos autores, cujo modelo está a seguir apresentado:
Eu/Nós, _________________________, autor(es) do trabalho intitulado _______________, submetido agora à apreciação da Clinical and Laboratorial Research in Dentistry, concordo(amos) que os autores retém o direitos autorais e garantem a revista o direito da primeira publicação, sendo o trabalho simultaneamente autorizado sob a Creative Commons Attribution License, que permite a outros compartilhar o artigo com reconhecimento da autoria do trabalho e publicação inicial nesta Revista. Aos autores será possibilitada a distribuição em separado da versão publicada do artigo, arranjos contratuais adicionais para a distribuição não-exclusiva da versão publicada (por exemplo, publicá-la em um repositório institucional ou publicação em livro), com o reconhecimento de sua publicação inicial nesta revista. Aos autores será permitido e encorajado publicar seu trabalho on-line (por exemplo, em repositórios institucionais ou em seu site) antes e durante o processo de envio, pois pode levar a intercâmbios produtivos, bem como a maior citação do trabalho publicado. (Veja The Effect of Open Access).
Data: ____/____/____Assinatura(s): _______________