Determination of the biological reactivity of ketoconazole in shampoo: draize eye test and cytotoxity test

Authors

  • Inara Staub Universidade Federal do Rio Grande do Sul; Faculdade de Farmácia
  • Áurea Silveira Cruz Instituto Adolfo Lutz; Seção de Culturas Celulares
  • Terezinha de Jesus Andreoli Pinto Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Farmácia
  • Elfrides Eva Scherman Schapoval Universidade Federal do Rio Grande do Sul; Faculdade de Farmácia
  • Ana Maria Bergold Universidade Federal do Rio Grande do Sul; Faculdade de Farmácia

DOI:

https://doi.org/10.1590/S1516-93322007000200017

Keywords:

Ketoconazole, Shampoo, Degradation products, Draize eye test, Cytotoxity test

Abstract

Ketoconazole is an antifungal agent and can be incorporated into several dosage forms, as an example it could be mentioned shampoos and creams. The aim of this work was to assess the biological reactivity in vivo (Draize eye test) and in vitro (cytotoxity test) of ketoconazole in shampoo degradeted under action of light. The formulation was exposed to UV-C (254 nm) radiation and two methods were used for the quantitative determination of ketoconazole: HPLC and microbiological assay. The results showed alteration in ketoconazole in presence of light - secondary peaks in chromatogram and decrease in antifungal activity - however, no alteration on the biological reactivity between ketoconazole shampoo and ketoconazole shampoo containing degradation products was observed.

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Published

2007-06-01

Issue

Vol. 43 No. 2 (2007)

Section

Original Papers

How to Cite

Determination of the biological reactivity of ketoconazole in shampoo: draize eye test and cytotoxity test. (2007). Revista Brasileira De Ciências Farmacêuticas, 43(2), 301-307. https://doi.org/10.1590/S1516-93322007000200017