Cecropia pachystachya Trécul: a promising ingredient for skin-whitening cosmetics

Pedro Henrique Santos de Freitas; Jessica Leiras Mota Conegundes; Monique Evangelista; Maycon Alcântara; Natalia Prado da Silva; Guilherme  Diniz; Fernanda Vilela; Ana Paula Duque; Antonia Ribeiro; Elita Scio

Authors

Pedro Henrique Santos de Freitas Laboratory of Bioactive Natural Products, Department of Biochemistry, Institute of Biological Science, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil https://orcid.org/0000-0002-6635-0471
Jessica Leiras Mota Conegundes Laboratory of Bioactive Natural Products, Department of Biochemistry, Institute of Biological Science, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Monique Evangelista Laboratory of Bioactive Natural Products, Department of Biochemistry, Institute of Biological Science, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Maycon Alcântara Laboratory of Bioactive Natural Products, Department of Biochemistry, Institute of Biological Science, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Natalia Prado da Silva Laboratory of Nanostructure Systems Development, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Guilherme Diniz Laboratory of Nanostructure Systems Development, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Fernanda Vilela Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Ana Paula Duque Laboratory of Bioactive Natural Products, Department of Biochemistry, Institute of Biological Science, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Antonia Ribeiro Laboratory of Bioactive Natural Products, Department of Biochemistry, Institute of Biological Science, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Elita Scio Laboratory of Bioactive Natural Products, Department of Biochemistry, Institute of Biological Science, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil https://orcid.org/0000-0002-5580-4845

Keywords:

Cecropia pachystachya; Glycolic extracts; Antioxidant activity; Tyrosinase inhibition; Skin whitening

Abstract

Because it promotes the lightening of pigment spots, tyrosinase inhibition is one of the mechanisms of depigmenting cosmetic products. Considering the adverse effects produced by synthetic depigmenting actives, the search for new therapeutic options is desirable, and plant extracts are possible candidates for hyperpigmentation treatment. Glycolic extracts of Cecropia pachystachya Trécul are, therefore, the focus of this study. Its chemical characterization, antioxidant activity, tyrosinase inhibition, and cell viability were evaluated. Glycolic extracts were obtained by macerating the leaves of C. pachystachya in grain alcohol and glycerin or propylene glycol. Both had a similar chemical constitution, the glycerin being more efficient in concentrating phenolic compounds and flavonoids. Analyses by UHPLC-MS detected quinic acid, chlorogenic acid isomers, proanthocyanidin dimers type B and C, catechin/epicatechin, orientin/isoorientin, isoorientin 2”-O-xyloside, vitexin/isovitexin, and rutin. 5-O-caffeoylquinic acid was then quantified was then quantified, with predominance in the extract produced with propylene glycol. These extracts showed a high antioxidant capacity by the method of DPPH, β-carotene, and nitric oxide. As for depigmenting activity, both extracts were able to inhibit tyrosinase. Cell viability assay also revealed that the extracts could safely be used in concentrations of ≤ 125 µg/mL. Thus, this study demonstrated for the first time that the glycolic extracts of C. pachystachya have promising chemical and biological characteristics for the development of a multifunctional cosmetic with antioxidant and tyrosinase-inhibition activities.

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Alves CQ, David JM, David JP, Bahia MV, Aguiar RM. Methods for determination of in vitro antioxidant activity for extracts and organic compounds. Quim Nova. 2010;33(10):2202-2210.

Aragão DMO, Guarize L, Lanini J, da Costa JC, Garcia RMG, Scio E. Hypoglycemic effects of Cecropia pachystachya in normal and alloxan-induced diabetic rats. J Ethnopharmacol. 2010;128(3):629-633.

Ardisson L, Godoy JS, Ferreira LAM, Stehmann JR, Brandão MGL. Preparation and characterization of propylene glicol extracts obtained from dried stem barks of Stryphnodendron adstringens (Mart.) Coville (barbatimão). Rev Bras Farmacogn. 2002;12(1):27-34.

Bigliani MC, Grondona E, Zunino PM, Ponce AA. Effects of Cecropia pachystachya and Larrea divaricata aqueous extracts in mice. Hum Exper Toxicol. 2010;29(7):601-606.

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci Technol. 1995;28(1):25-30.

Challice J, Markham UR. Techniques of Flavonoid Identification. Biol Plant. 1984;26:302.

Chang TS. An updated review of tyrosinase inhibitors. Int J Mol Sci. 2009;10(6):2440-2475.

Chen G, Li X, Saleri F, Guo M. Analysis of flavonoids in Rhamnus davurica and its antiproliferative activities. Molecules. 2016;21(10):1275.

Conforti F, Rigano D, Menichini F, Loizzo MR, Senatore F. Protection against neurodegenerative diseases of Iris pseudopumila extracts and their constituents. Fitoterapia. 2009;80(1):62-67.

Costa GM, Schenkel EP, Reginatto FH. Chemical and pharmacological aspects of the genus Cecropia Nat. Prod. Commun. 2011;6(6):913-920.

Duque APDN, Pinto NDCC, Mendes RDF, Da Silva JM, Aragão DMDO, Castañon MCMN, et al. In vivo wound healing activity of gels containing Cecropia pachystachya leaves. J Pharm Pharmacol. 2016;68(1):128-138.

Fernandes MF, Conegundes JLM, Pinto NDCC, Oliveira LG De, Aguiar JAK De, Souza-Fagundes EM, et al. Cecropia pachystachya leaves present potential to be used as new ingredient for antiaging dermocosmetics. Evidence-based Complement Altern Med. 2019;2019: 8263934.

Folin O, Ciocalteu V. On tyrosine and tryptophane determinations in proteins. J Biol Chem. 1927;73(2):627-650.

Fonseca-Santos B, Corrêa M A, Chorilli M. Sustainability, natural and organic cosmetics: Consumer, products, efficacy, toxicological and regulatory considerations. Braz J Pharm Sci. 2015;51(1):17-26.

Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem. 1982;126(1):131-138.

Ikino JK, Nunes DH, da Silva VPM, Sens MM, Fröde TS. Melasma and assessment of the quality of life in Brazilian women. An Bras Dermatol. 2015;90(2):196-200.

ISO 10993-5: Biological evaluation of medical devices Part 5: Tests for cytotoxicity: in vitro methods (2009). Available from: Available from: https://www.iso.org/standard/36406.html (Accessed 11 June 2020).

» https://www.iso.org/standard/36406.html

Itoh K, Hirata N, Masuda M, Naruto S, Murata K, Wakabayashi K, et al. Inhibitory effects of Citrus hassaku extract and its flavanone glycosides on melanogenesis. Biol Pharm Bull. 2009;32(3):410-415.

Khatib S, Nerya O, Musa R, Shmuel M, Tamir S, Vaya J. Chalcones as potent tyrosinase inhibitors: The importance of a 2,4-substituted resorcinol moiety. Bioorganic Med Chem. 2005;13(2):433-441.

Kubo I, Kinst-Hori I, Chaudhuri SK, Kubo Y, Sánchez Y, Ogura T. Flavonols from Heterotheca inuloides: Tyrosinase inhibitory activity and structural criteria. Bioorganic Med Chem . 2000;8(7):1749-1755.

Lago JHG, Toledo-Arruda AC, Mernak M, Barrosa KH, Martins MA, Tibério IFLC, et al. Structure-Activity association of flavonoids in lung diseases. Molecules . 2014;19(3):3570-3595.

Machado CD, Klider LM, Tirloni CAS, Marques AAM, Lorençone BR, Batista LP, et al. Ethnopharmacological investigations of the leaves of Cecropia pachystachya Trécul (Urticaceae): A native Brazilian tree species. J Ethnopharmacol . 2021;270(2021):113740.

Marco GJ. A rapid method for evaluation of antioxidants. J Am Oil Chem Soc. 1968;45(9):594-598.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63.

OECD Test Guideline No. 439 - In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method (2010). Available from: Available from: https://ecvam-dbalm.jrc.ec.europa.eu/methods-and-protocols/protocols/protocol/episkin-skin- irritation-test15min-42-hours/protocol-no/131/key/p_1419 (Accessed 11 June 2020).

» https://ecvam-dbalm.jrc.ec.europa.eu/methods-and-protocols/protocols/protocol/episkin-skin- irritation-test15min-42-hours/protocol-no/131/key/p_1419

Opperman L, De Kock M, Klaasen J, Rahiman F. Tyrosinase and melanogenesis inhibition by indigenous African plants: A review. Cosmetics. 2020;7(3):60.

Ortmann CF, Abelaira HM, Réus GZ, Ignácio ZM, Chaves VC, dos Santos TC, et al. LC/QTOF profile and preliminary stability studies of an enriched flavonoid fraction of Cecropia pachystachya Trécul leaves with potential antidepressant- like activity. Biomed Chromatogr. 2017;31(11):E3982.

Pacheco NR, Pinto NDCC, Mello Da Silva J, Mendes RDF, Costa JDC Da, Aragão DMDO, et al. Cecropia pachystachya: A species with expressive in vivo topical anti- inflammatory and in vitro antioxidant effects. BioMed Res. Int. 2014;2014:301294.

Padmawar, AR, Bhadoriya, U. Glycol and Glycerin: Pivotal Role in Herbal Industry as Solvent/Co-Solvent. World J Pharm Med. 2018;4(5):153-155.

Palomino García LR, Del Bianchi VL. Antioxidant capacity in coffee industry residues. Braz J Food Technol. 2015;18(4):307-313.

Plazas M, Prohens J, Cuñat AN, Vilanova S, Gramazio P, Herraiz FJ, et al. Reducing capacity, chlorogenic acid content and biological activity in a collection of scarlet (Solanum aethiopicum) and Gboma (S. macrocarpon) eggplants. Int J Mol Sci . 2014;15(10):17221-17241.

Poupard P, Sanoner P, Baron A, Renard CMGC, Guyot S. Characterization of procyanidin B2 oxidation products in an apple juice model solution and confirmation of their presence in apple juice by high-performance liquid chromatography coupled to electrospray ion trap mass spectrometry. J Mass Spectrom. 2011;46(11):1186-1197.

Rivera-Mondragón A, Bijttebier S, Tuenter E, Custers D, Ortíz OO, Pieters L, et al. Phytochemical characterization and comparative studies of four Cecropia species collected in Panama using multivariate data analysis. Sci Rep. 2019;9:1763.

da Silva Mathias M, Rodrigues de Oliveira R. Differentiation of the phenolic chemical profiles of Cecropia pachystachya and Cecropia hololeuca Phytochem Anal. 2019;30(1):73-82.

Singh BK, Park SH, Lee HB, Goo YA, Kim HS, Cho SH, et al. Kojic acid peptide: A new compound with anti-tyrosinase potential. Ann Dermatol. 2016;28(5):555-561.

Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorun T. Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutat Res - Fundam Mol Mech Mutagen. 2005;579(1-2):200-213.

Taofiq O, Rodrigues F, Barros L, Barreiro MF, Ferreira ICFR, Oliveira MBPP. Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies. Food Chem Toxicol. 2019;127:228-236.

Verstraeten SV, Keen CL, Schmitz HH, Fraga CG, Oteiza PI. Flavan-3-ols and procyanidins protect liposomes against lipid oxidation and disruption of the bilayer structure. Free Radic Biol Med. 2003;34(1):84-92.

Xie P jun, Huang L xin, Zhang C hong, Ding S sha, Deng Y jun, Wang X jie. Skin-care effects of dandelion leaf extract and stem extract: Antioxidant properties, tyrosinase inhibitory and molecular docking simulations. Ind Crops Prod. 2018;111:238-246.

Yazbek PB, Matta P, Passero LF, Santos GD, Braga S, Assunção L, et al. Plants utilized as medicines by residents of Quilombo da Fazenda, Núcleo Picinguaba, Ubatuba, São Paulo, Brazil: A participatory survey. J Ethnopharmacol . 2019;244:112123.

Cecropia pachystachya Trécul

a promising ingredient for skin-whitening cosmetics

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