Authentication of Brazilian Ginseng using Bar-HRM analysis

Authors

  • Francine Alves Nogueira de Almeida Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brazil https://orcid.org/0000-0002-1633-9477
  • Pablo Viana Oliveira Programa de Pós-Graduação em Biotecnologia http://orcid.org/0000-0002-1785-178X
  • Natan Silva Matos Departamento de Farmácia e Nutrição, Universidade Federal do Espírito Santo, Alegre, ES, Brazil
  • Verônica Luiza Luiza Silveira Fialho Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brazil
  • Magda Delorence Lugon Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brazil http://orcid.org/0000-0001-8156-7903
  • Maria do Carmo Vieira Faculdade de Ciências Agrárias, Universidade Federal de Grandes Dourados, MS, Brazil http://orcid.org/0000-0001-7047-3848
  • Márcia Flores da Silva Ferreira Programa de Pós-Graduação em Genética e Melhoramento, Universidade Federal do Espírito Santo, Alegre, ES, Brazil http://orcid.org/0000-0003-1541-6634
  • Greiciane Gaburro Paneto Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Espírito Santo, Vitória, ES, Brazil ,Departamento de Farmácia e Nutrição, Universidade Federal do Espírito Santo, Alegre, ES, Brazil http://orcid.org/0000-0001-8035-4199

Keywords:

Amaranthaceae;, Eriantha;, Glomerata;, Hebanthe;, Pfaffia.

Abstract

Hebanthe eriantha (Martius) Kuntze and Pfaffia glomerata (Spreng) Pedersen are medicinal plants popularly known as “Brazilian Ginseng” due to their similarity to Panax ginseng. In Brazil, they are sold as the same herb, despite their different pharmacological and toxicological properties. The morphological identification is difficult, which facilitates their adulteration. We report the application of the Barcode DNA High-Resolution Melting (Bar-HRM) using matK gene to differentiate both species in samples sold in the Brazilian market. Using the proposed method, we could discriminate and identify both species. Bar-HRM analysis allowed discriminating and identifying both species. It allowed the identification of H. eriantha and P. glomerata in 43.6% and 56.4% of the amplified samples, respectively. Of these, only seven samples were authenticated and, in 71.4% of the cases, adulterated. We concluded that Bar-HRM has proven to be a fast alternative method to authenticate plants under the common name “Brazilian Ginseng”.

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Published

2023-08-28

Issue

Vol. 59 (2023)

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Article

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How to Cite

Authentication of Brazilian Ginseng using Bar-HRM analysis. (2023). Brazilian Journal of Pharmaceutical Sciences, 59, 10. https://www.periodicos.usp.br/bjps/article/view/220151

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