Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH· reduction

Authors

  • Diogo Pineda Rivelli Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Análises Clínicas e Toxicológicas
  • Vanessa Vitoriano da Silva Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Análises Clínicas e Toxicológicas
  • Cristina Dislich Ropke Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Análises Clínicas e Toxicológicas
  • Denise Varella Miranda Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Análises Clínicas e Toxicológicas
  • Rebeca Leite Almeida Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Análises Clínicas e Toxicológicas
  • Tania Cristina Higashi Sawada Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Análises Clínicas e Toxicológicas
  • Silvia Berlanga de Moraes Barros Universidade de São Paulo; Faculdade de Ciências Farmacêuticas; Departamento de Análises Clínicas e Toxicológicas

DOI:

https://doi.org/10.1590/S1516-93322007000200007

Keywords:

Chlorogenic acid, Caffeic acid, Caffeine, Antioxidant, DPPH, Ilex paraguariensis

Abstract

A new high performance liquid chromatographic method has been established for simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis. Analytical curves showed good linear regression in the concentration ranges 0.49-7.8 µg/mL for chlorogenic acid, 0.25-3.9 µg/mL for caffeic acid and 0.244-7.8 µg/mL for caffeine. Reduction of the DPPH· radical was used to determine the antioxidant capacity of the extracts. Our method for the simultaneous determination of chlorogenic acid, caffeic acid and caffeine was highly sensitive, having lower detection and quantitation limits than other papers that used similar methodology.

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Published

2007-06-01

Issue

Vol. 43 No. 2 (2007)

Section

Original Papers

How to Cite

Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH· reduction. (2007). Revista Brasileira De Ciências Farmacêuticas, 43(2), 215-222. https://doi.org/10.1590/S1516-93322007000200007